G8160

Sigma

 

Granulocyte Colony-Stimulating Factor from mouse

recombinant, expressed in Escherichia coli, cell culture tested

Synonym:G-CSF, Granulocyte Colony-Stimulating Factor from mouse
MDL number:MFCD00166475

Description

Analysis NoteThe biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.
Biochem/physiol ActionsGranulocyte-colony stimulating factor is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G0 period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.
Physical formLyophilized from 20 mM acetic acid containing 250 μg bovine serum albumin

Properties

biological source from mouse
recombinantexpressed in Escherichia coli
assay>97% (SDS-PAGE)
formlyophilized powder
potency0.005-0.05 ng/mL ED50/EC50
mol wtmol wt 19 kDa (179 amino acids including N-terminal methionine)
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.−20°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3

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 G5296, Anti-Granulocyte Colony Stimulating Factor antibody produced in goat
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References

referenceMatthew, W.D., and Patterson, P. Cold Spring Harb. Symp. Quant. Biol. 48, 625, (1983)
 Nicola, N.A., Nicola, N.A., ed. Guidebook to Cytokines and Their Receptors NY, NY , (1994), 171
 Risau, W., et al. EMBO J. 5, 3179, (1986)
 Smith, L.R., et al. Immunogenetics 39, 80, (1994)
 Seulberger, H., et al. EMBO J. 9, 2151, (1990)
 Quesniaux, V.F.J., Jones, T.C. The Cytokine Handbook 3rd ed., San Diego, CA , (1998), 637
 Callard, R., Gearing, A. The Cytokine Facts Book New York, NY , (1994)
 Kelso, A., Cytokines: principles and prospects. Cell Biol. 76, 300, (1998)
 Guthridge, M., Mechanism of activation of the GM-CF, IL-3, and IL-5 family of receptors. Stem Cells 16, 301, (1998)
 Heim, M., The Jak-STAT pathway: cytokine signaling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19, 75, (1999)
 Skoda, R., Specificity of signaling by hematopoietic cytokin receptors: instructive versus permissive effects. J. Recept. Signal Transduct. Res. 19, 741, (1999)